Nanopore Sequencing Technology and Tools: Computational Analysis of the Current State, Bottlenecks and Future Directions
نویسندگان
چکیده
Nanopore sequencing [1-4], a promising single-molecule DNA sequencing technology, exhibits many attractive qualities and, in time, could potentially surpass current sequencing technologies. Nanopore sequencing promises higher throughput, lower cost, and increased read length, and it does not require a prior amplification step. Nanopore sequencers rely solely on the electrochemical structure of the different nucleotides for identification and measure the change in the ionic current as long strands of DNA (ssDNA) pass through the nano-scale protein pores.
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